Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells

Reinhold, Dirk and Guttek, Karina and Reddig, Annika and Voss, Linda and Schubert, Claudia and Kahlfuss, Sascha and Grüngreiff, Kurt and Schraven, Burkhart and Reinhold, Annegret (2021) Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells. Biomedicines, 9 (3). p. 246. ISSN 2227-9059

[thumbnail of biomedicines-09-00246-v2.pdf] Text
biomedicines-09-00246-v2.pdf - Published Version

Download (17MB)

Abstract

T cell activation mediates immunity to pathogens. On the flipside, T cells are also involved in pathological immune responses during chronic autoimmune diseases. We recently reported that zinc aspartate, a registered drug with high bioavailability, dose-dependently inhibits T cell activation and Th1/Th2/Th17 cytokine production of stimulated human and mouse T cells. To understand the suppressive effect of zinc on T cell function, we here investigated the influence of zinc aspartate on human T cells focusing on the secretion of immunosuppressive cytokines, induction of apoptosis, and caspase 3/7 activity. To this end, we monitored either freshly stimulated or pre-activated human T cells in the presence of zinc aspartate from 40–140 µM over a period of 72 h. Under both experimental conditions, we observed a dose-dependent suppression of human T cell proliferation. While IL-1ra, latent TGF-β1, and IL-10 were dose-dependently reduced, we, unexpectedly, detected elevated levels of IL-16 upon zinc supplementation. In addition, the number of cells with active caspase 3/7 and, consecutively, the amount of cells undergoing apoptosis, steadily increased at zinc aspartate concentrations exceeding 100 µM. Taken together, our findings suggest that zinc aspartate impairs T cell fitness and might be beneficial for the treatment of T cell-mediated autoimmune diseases.

Item Type: Article
Subjects: Open Article Repository > Biological Science
Depositing User: Unnamed user with email support@openarticledepository.com
Date Deposited: 16 Jan 2023 08:56
Last Modified: 29 Jun 2024 11:03
URI: http://journal.251news.co.in/id/eprint/146

Actions (login required)

View Item
View Item