Estradiol and/or Ibandronate Therapy Ameliorates Oxidative Status in Livers of Ovariectomized Rats

Background: Overiectomized rats suffer from osteoporosis that mainly results from oxidative stress (OS). Studies revealed that the levels of antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase, catalase (CAT), and glutathione reductase could be used to determine and follow-up OP. The anti-oxidant activity of anti-osteoporotic drugs needs further investigations to be proved to add more confidence in the ability of these drugs to control the disease.
Objective: To investigate the possible anti-oxidant effect of treatment with ibandronate, a highly potent nitrogen-containing bisphosphonate, on activities of catalase and glutathione peroxidase anti-oxidant enzymes, thiobarbituric reactive substance (TBARS), as a marker of lipid peroxidation and DEPPD free radicals in liver homogenates of ovariectomized rats.
Methods: Fifty adult female albino rats were divided into five equal groups ( n=10 rats): Group (1): served as control injected with saline, 2nd group: rats were subjected to ovariectomy, 3rd group: rats were exposed to ovariectomy and treated with estradiol, 4th group: were ovariectomized and treated with ibandronate and 5th group: were ovariectomized and treated with both estradiol and ibandronate. Duration of therapy with either drug was 12 weeks.
Results: Estradiol alone or in combination with ibandronate to ovariectomized rats showed significant increase in activities of anti-oxidant enzymes. Both drugs reduces hepatic TBARS and DEPPD free radicals in hepatic homogenates in this model of OP.
Conclusion: The present study can conclude that ovariectomy leading to oxidative changes liver of tested rats. Co-administration of estradiol and ibandronate provides a kind of protection against alterations in anti-oxidative/oxidative balance. The results support the hypothesis stated by experimental studies that anti-osteoporotic treatment could also possess anti-oxidative protective property.