Intracytoplasmic Sperm Injection (ICSI) with Fresh versus Cryopreserved Testicular Sperm in Egyptians Non-Obstructive Azoospermic Patients

Objective (s): Assessment of fertilization rate, grading of embryos, blastocyst formation rate,arrested 2PN and clinical pregnancy outcome during intracytoplasmic sperm injection (ICSI) in fresh testicular sperm versus frozen–thawed testicular sperm in Egyptians non-obstructive azoospermia.
Design: Prospective study.
Setting: assisted reproduction unit at the ELITE fertility care center,Heliopolis, Cairo, Egypt between October 2017 and September 2019.
Subject: evaluate testicular sperm selection technique using fresh and thawing testicular sperm extraction (TESE) in intracytoplasmic sperm injection (ICSI). The study population consisted of 97 couples. Results: The study population of 97 couples who referred to ICSI divided into 2 groups, fresh testicular sperm and frozen–thawed testicular sperm, including complete dissecting analyses, flow up and comparison between the changes between two groups during ICSI outcome. Conclusion: the present study show significant differences in fertilization rate between two groups, and no significant differences in grading of embryos, blastocyst formation rate,arrested 2PN and clinical pregnancy rate.